Sample of analysis of a demo short reads file
Columns:
1. Fasta ID
2. Number of Short Read
3. Frame (one of six possible reading frames (see Swissprot translation link)
4. Location of the starting point of the SP on the nucleotide chain
5. SP - Specific Peptide
6. EC number (At level 3 or 4)
7. Original nucleotide short read sequence
Fasta ID | Short Read # | Frame | Location | SP | EC | Short Read |
---|---|---|---|---|---|---|
>SR1 | 1 | 1 | 45 | VGFDVLGAA | 2.7.1.39 | ATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGG |
>SR1 | 1 | 1 | 15 | YAPASSAN | 2.7.1.39 | ATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGG |
>SR2 | 2 | 2 | 48 | NIVYQCW | 2.7.1.39 | CAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAG |
>SR2 | 2 | 2 | 3 | NLGRFADKLP | 2.7.1.39 | CAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAG |